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Authors
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Pilz, M. ; Kratzmüller, Th. ; Eberth, G. ; Werner, C.
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Title
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Imaging Adsorbed Beta-2-Microglobulin Layers on Hydrophobic and Hydrophilic Substrates by Atomic Force Microscopy
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Date
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31.12.2000
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Number
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9190
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Abstract
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Adsorbed layers of beta-2-microglobulin on unmodified and methylated silicon wafers were studied by atomic force microscopy and by spectroscopic ellipsometry. The results provide evidence that hydrophobicity of the substrate increases the maximum adsorbed amount of beta-2-microglobulin and the resistance of the adsorbed protein to displacement from the interface by competing fibrinogen, respectively. The observed variations in displacement behavior of adsorbed beta-2-microglobulin were interpreted based on information on the layer structure gained by atomic force microscopy. More compact and relatively smooth protein layers were formed on the hydrophobic surface corresponding to lower displacement by fibrinogen.
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Publisher
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Single Molecules
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Wikidata
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Citation
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Single Molecules 1 (2000) 171-172
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DOI
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https://doi.org/10.1002/1438-5171(200006)1:2<171::AID-SIMO171>3.0.CO;2-S
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