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Title 3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK
Date 23.08.2019
Number 57002
Abstract Mechanical properties of cancer cells and their microenvironment contribute to breast cancer progression. While mechanosensing has been extensively studied using two-dimensional (2D) substrates, much less is known about it in a physiologically more relevant 3D context. Here we demonstrate that breast cancer tumor spheroids, growing in 3D polyethylene glycol-heparin hydrogels, are sensitive to their environment stiffness. During tumor spheroid growth, compressive stresses of up to 2 kPa built up, as quantitated using elastic polymer beads as stress sensors. Atomic force microscopy (AFM) revealed that tumor spheroid stiffness increased with hydrogel stiffness. Also, constituent cell stiffness increased in a ROCK- and F-actin-dependent manner. Increased hydrogel stiffness correlated with attenuated tumor spheroid growth, a higher proportion of cells in G0/G1 phase and elevated levels of the cyclin-dependent kinase inhibitor p21. Drug-mediated ROCK inhibition reversed not only cell stiffening upon culture in stiff hydrogels but also increased tumor spheroid growth. Taken together, we reveal here a mechanism by which the growth of a tumor spheroid can be regulated via cytoskeleton rearrangements in response to its mechanoenvironment. Thus, our findings contribute to a better understanding of how cancer cells react to compressive stress when growing under confinement in stiff environments and provide the basis for a more in-depth exploration of the underlying mechanosensory response.
URL https://doi.org/10.1101/586784
Publisher Advanced Biosystems
Identifier
Citation Advanced Biosystems 3 (2019) 1900128
DOI https://doi.org/10.1101/586784
Authors Taubenberger, A. ; Girardo, S. ; Träber, N. ; Fischer-Friedrich, E. ; Kräter, M. ; Wagner, K. ; Kurth, T. ; Richter, I. ; Binner, M. ; Hahn, D. ; Freudenberg, U. ; Werner, C. ; Guck, J.
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