Control of protein adsorption and cell adhesion by mixed polymer brushes made by the "grafting-to" approach
Proteins at Interfaces III State of the Art, Horbett, T. ; Brash, J.L. ; Norde, W., ed.
|Abstract||The capability of mixed polymer brush systems to modify and switch physico-chemical properties of biointerfaces is demonstrated in this paper. Mixed brushes were composed of the temperature sensitive polymer poly(N-isopropyl acrylamide) (PNIPAAM) and two polyelectrolytes, poly(2-vinylpyridine) (P2VP) and poly(acrylic acid) (PAA). Using these polymers binary brushes were prepared, the first one consisting of PNIPAAM P2VP representing a system sensitive for temperature and pH changes, and the second one consisting of two polyelectrolytes (P2VP-PAA) showing sensitivity to pH changes of the surrounding fluid. Selected aspects concerning the environmental sensitivity as well as the protein adsorption affinity of these brushes are discussed. The stimuli-response towards temperature (PNIPAAm-P2VP) and pH (P2VP-PAA) of these binary polymer brushes was utilized to switch the adsorbed amount of protein and finally to regulate cell adhesion at brush coated surfaces. <br /><br />Proteins at Interfaces III<br />ISBN 978-0-8412-2796-5|
|Publisher||American Chemical Society|
|Citation||American Chemical Society 1120 (2012) Chapter 8, 179-193|
|Authors||Bittrich, E. ; Burkert, S. ; Eichhorn, K.-J. ; Stamm, M. ; Uhlmann, P.|