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Authors
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Zimmermann, R. ; Hentschel, C. ; Schrön, F. ; Moedder, D. ; Büttner, T. ; Atallah, P. ; Wegener, T. ; Howitz, S. ; Werner, C.
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Title
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High resolution bioprinting of multi-component hydrogels
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Date
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29.07.2019
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Number
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55546
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Abstract
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Materials capable of directing cell fate by providing spatially-graded mechanical and biomolecular cues are critically important in the reconstitution of living matter. Herein, we report a multi-component inkjet bioprinting method that allows for spatially varying composition and network properties in cell-instructive glycosaminoglycan (GAG)-based biohybrid and pure poly(ethylene glycol) hydrogels with unprecedented (50 µm) resolution. The principle relies on the covalent crosslinking of different polymeric precursors through a very rapid bio-orthogonal Michael type addition scheme adjusted in ways to occur during the fusion of bio-ink droplets prior to and upon contact with the target. Exemplary data show that chemotactic molecular gradients produced by this approach within printed GAG-gels of defined zonal architecture can effectively direct migratory activity and morphogenesis of embedded human bone-marrow derived mesenchymal stem cells. The introduced methodology is expected to enable a new, holistic level of control over reductionistic tissue and organoid models.
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Publisher
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Biofabrication
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Wikidata
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Citation
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Biofabrication 11 (2019) 045008
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DOI
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https://doi.org/10.1088/1758-5090/AB2AA1
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Tags
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