Authors Chen, H. ; Li, X. ; Zhao, Y. ; Li, J. ; Chen, J. ; Yang, P. ; Maitz, M.F. ; Huang, N.
Title Construction of a multifunctional coating consisting of phospholipids and endothelial progenitor cell-specific peptides on titanium substrates
Date 30.08.2015
Number 47322
Abstract A phospholipid/peptide polymer (PMMDP) with phosphorylcholine groups, endothelial progenitor cell (EPC)-specific peptides and catechol groups was anchored onto a titanium (Ti) surface to fabricate a biomimetic multifunctional surface. The PMMDP coating was characterized by X-ray photoelectron spectroscopy (XPS), water contact angle measurements and atomic force microscopy (AFM), respectively. The amount of PMMDP coating on the Ti surface was quantified by using the quartz crystal microbalance with dissipation (QCM-D). Interactions between blood components and the coated and bare Ti substrates were evaluated by platelet adhesion and activation assays and fibrinogen denaturation test using platelet rich plasma (PRP). The results revealed that the PMMDP-modified surface inhibited fibrinogen denaturation and reduced platelet adhesion and activation. EPC cell culture on the PMMDP-modified surface showed increased adhesion and proliferation of EPCs when compared to the cells cultured on untreated Ti surface. The inhibition of fibrinogen denaturation and platelet adhesion and support of EPCs attachment and proliferation indicated that this coating might be beneficial for future applications in blood-contacting implants, such as vascular stents. (C) 2015 Elsevier B.V. All rights reserved.
Publisher Applied Surface Science
Citation Applied Surface Science 347 (2015) 169-177
Tags titanium phospholipid specific peptide endothelial progenitor cells biomimetic multifunctional coating self-endothelialization nitric-oxide surface blood immobilization adhesion antibody functionalization mobilization nanomatrix

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