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Authors
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Ohgo, K. ; Bagusat, F. ; Asakura, T. ; Scheler, U.
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Title
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Investigation of structural transition of regenerated silk fibroin aqueous solution by rheo-NMR spectroscopy
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Date
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03.04.2008
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Number
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15599
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Abstract
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In this study we applied Rheo-NMR to investigate the structural change of Bombyx mori silk fibroin in aqueous solution under shear. Monitoring the time dependence of 1H solution NMR spectra of silk fibroin subjected to constant shear strain, signal intensities of random coil decreased suddenly during shear while peaks from -sheet structure did not arise in the solution spectra. After these experiments, an aggregate of silk was found in the Couette flow cell and its secondary structure was determined as -sheet by 13C solid-state NMR. In conclusion the moderate shear applied here triggered the change in the secondary structure.
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Publisher
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Journal of the American Chemical Society
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Wikidata
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Citation
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Journal of the American Chemical Society 130 (2008) 4182-4186
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DOI
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https://doi.org/10.1021/ja710011d
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Tags
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residue carbonyl carbons bombyx-mori silkworm beta-turn structure solid-state nmr chemical-shifts middle division peptides refinement scattering resonance
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