Authors Sperling, C. ; Houska, M. ; Brynda, E. ; Streller, U. ; Werner, C.
Title In vitro hemocompatibility of albumin-heparin multilayer coatings on polyethersulfone prepared by the layer-by-layer technique
Date 01.03.2006
Number 13381
Abstract Polyethersulfone foils (PES)&nbsp;-&nbsp;a unique material for blood purification membranes&nbsp;-&nbsp;were coated with a multilayer assembly of heparin (unfractionated or high anticoagulant activity fraction heparin) and albumin (albumin-heparin coatings), or with a multilayer of albumin (albumin coating), using the layer-by-layer technique. The coatings combine advantages of albumin (reduction of nonspecific interactions) and heparin (specific interactions with blood coagulation proteins). The differences between the two heparins, while significant for their biological activity, had only a minor effect on the multilayer assembly with albumin monitored <I>in situ</I> by reflection infrared spectroscopy (FTIR MIRS). Uncoated as well as modified PES surfaces were evaluated using an <I>in vitro</I> assay with freshly drawn, slightly heparinized (1.5 IU heparin/mL) human whole blood. The blood was circulated<br /> with a roller pump over the sample surfaces in shear flow across rectangular slit channels (<IMGBORDER="0"ALIGN="ABSMIDDLE"src="nueq001">app. 6 mL/min and <IMGBORDER="0"ALIGN="ABSMIDDLE"src="nueq001">120 s<SUP>-1</SUP>) for 1.5 h at 37°C. All coatings effectively reduced platelet adhesion and activation according to the PF4 release. The activation of coagulation evaluated as TAT generation was significantly lowered for the coating composed of albumin and high activity heparin. A further beneficial effect of the heparin containing coatings was reduced complement activation as determined by different complement fragments. &copy; 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Publisher Journal of Biomedical Materials Research Part A
Citation Journal of Biomedical Materials Research Part A 76 (2006) 681-689

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