Authors Sperling, C. ; Schweiß, R. ; Streller, U. ; Werner, C.
Title In vitro hemocompatibility of self-assembled monolayers displaying various functional groups
Date 30.08.2005
Number 12523
Abstract Self-assembled monolayers (SAMs) of alkanethiols with various terminating groups (&#x2013;OH, &#x2013;CH<sub>3</sub>, &#x2013;COOH) and binary mixtures of these alkanethiols were studied with respect to their hemocompatibility in vitro by means of freshly taken human whole blood. The set of smooth monomolecular films with graded surface characteristics was applied to scrutinize hypotheses on the impact of surface chemical&#x2013;physical properties on distinct blood activation cascades, i.e. to analyze &#x2013;OH surface groups vs. complement activation, acidic surface sites vs. contact activation/coagulation and surface hydrophobicity vs. thrombogenicity. Blood and model surfaces were analyzed after incubation for the related hemocompatibility parameters. Our results show that the adhesion of leukocytes is abolished on a &#x2013;CH<sub>3</sub> surface and greatly enhanced on surfaces with &#x2013;OH groups. The opposite was detected for the adhesion of platelets. A strong correlation between the activation of the co<br />mplement system and the adhesion of leukocytes with the content of &#x2013;OH groups could be observed. The contact activation for hydrophilic surfaces was found to scale with the amount of acidic surface sites. However, the coagulation and platelet activation did not simply correlate with any surface property and were therefore concluded to be determined by a superposition of contact activation and platelet adhesion.
Publisher Biomaterials
Citation Biomaterials 26 (2005) 6547-6557
Tags MBC_Blood_Activation

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