Authors Gouzy, M.-F. ; Sperling, C. ; Salchert, K. ; Pompe, T. ; Streller, U. ; Uhlmann, P. ; Rauwolf, C. ; Simon, F. ; Böhme, F. ; Voit, B. ; Werner, C.
Title In vitro blood compatibility of polymeric biomaterials through covalent immobilization of an amidine derivative
Date 12.03.2004
Number 11501
Abstract We present a surface coating with anticoagulant characteristics showing significantly reduced coagulation activation. The synthesis of a monomeric conjugate containing a benzamidine moiety was carried out and its inhibitory activity against human thrombin, the key enzyme of the blood coagulation cascade, was determined using a chromogenic assay. Based on that, low-thrombogenic interfaces were prepared by covalent attachment of this low-molecular weight thrombin inhibitor on poly(octadecene-<i>alt</i>-maleic anhydride) copolymer thin films and characterized using ellipsometry, XPS and dynamic contact angle measurements. The in vitro hemocompatibility tests using freshly drawn human whole blood showed, in agreement with the SEM images, t<br />hat a PO-MA film modified with a benzamidine moiety using a PEG spacer decreased the activation of coagulation, platelets and the complement system. The decreased protein adsorption, in addition to the specific inhibition of thrombin, effectively enhanced the short-term hemocompatibility characteristics.
Publisher Biomaterials
Citation Biomaterials 25 (2004) 3493-3501
Tags maleic anhydride copolymer surface coating thrombin inhibitor immobilization blood compatibility human thrombomodulin thrombin inhibitor protein adsorption serine proteases surfaces hemocompatibility polyurethane benzamidine drug

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