Authors
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Gorzkiewicz, M. ; Sztandera, K. ; Jatczak-Pawlik, I. ; Zinke, R. ; Appelhans, D. ; Klajnert-Maculewicz, B. ; Pulaski, L.
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Title
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Terminal sugar moiety determines immunomodulatory properties of poly(propyleneimine) glycodendrimers
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Date
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23.03.2018
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Number
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54588
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Abstract
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Poly(propyleneimine) dendrimers fully surface-modified with disaccharide moieties (maltose, cellobiose, and lactose) designed to mimic natural lectin receptor ligands were tested for their bioactivity in two myeloid cell lines: THP-1 and HL-60. Depending on the sugar modification, we observed variable activation of NF-·B, AP-1, and NF-AT signaling pathways: lactose-coated dendrimers had the strongest impact on marker gene expression and most signaling events with the notable exception of NF-·B activation in THP-1 cells. The two cell lines showed an overall similar pattern of transcription factor and gene expression activation upon treatment with glycodendrimers, suggesting the involvement of galectin and C-type lectin receptor types. An important result of this action was the overexpression of CD40 and IL8 genes, potentially leading to an activated, proinflammatory phenotype in the monocyte/macrophage cell lineage. These pharmacodynamic characteristics of glycodendrimers need to be taken into account during their pharmaceutical applications both in drug delivery and direct immunomodulation.
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Publisher
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Biomacromolecules
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Wikidata
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Citation
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Biomacromolecules 19 (2018) 1562-1572
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DOI
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https://doi.org/10.1021/acs.biomac.8b00168
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Tags
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