Authors
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Liu, S. ; Liu, T. ; Chen, J. ; Maitz, M.F. ; Chen, C. ; Huang, N.
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Title
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Influence of a layer-by layer-assembled multilayer of anti-CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface
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Date
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11.03.2013
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Number
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37563
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Abstract
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The endothelialization of the metal surface of vascular stents came into focus as a new method for improving the biocompatibility of intravascular stents. This article has its focus on building a biofunctional layer on the activated titanium surface with anti-CD34 antibody, vascular endothelial growth factor (VEGF), and heparin by a layer-by-layer (LBL) self-assembly technique, to promote the endothelialization of the surface. When compared with titanium surface, the number of adhered endothelial progenitor cells (EPCs) on LBLs increased 47.1% after 5 days culture, meanwhile the proliferation rate of EPCs on LBLs during 5 days culture also exhibit significantly improvement. The results of blood compatibility evaluation clearly show that the LBLs reduce the number of adhered and activated blood platelets (adhered: Ti 83% vs. LBL <20% and activated: Ti 57% vs. LBL 19%), and the activated partial thromboplastin time of the LBL surface prolonged about 20 s in compared with platelet-poor plasma. The assembled LBL thus improves the thromboresistance of the titanium surface. The presented biofunctional multilayer of anti-CD34, VEGF, and heparin on titanium can significantly improve the blood compatibility and the endothelialization of a medical device. The biofunctional layer supports generation of a new endothelium onto titanium surface by capturing EPCs and oriented differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
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Publisher
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Journal of Biomedical Materials Research Part A
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Wikidata
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Citation
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Journal of Biomedical Materials Research Part A 101 (2013) 1144-1157
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DOI
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https://doi.org/10.1002/jbm.a.34392
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Tags
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