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Zieris, A. ; Prokoph, S. ; Welzel, P. ; Grimmer, M. ; Levental, K. ; Panyanuwat, W. ; Freudenberg, U. ; Werner, C.
Analytical approaches to uptake and release of hydrogel-associated FGF-2

Strategies to control the delivery of growth factors are critically important in the design of advanced biomaterials. In this study we investigated the binding and release of fibroblast growth factor 2 (FGF-2) to/from a biohybrid hydrogel matrix by four independent analytical methods: radioisotope and fluorescence labeling, amino acid analysis and Enzyme-Linked Immunosorbent Assays (ELISA). The compared analyses provided qualitatively similar uptake characteristics while the results of the FGF-2 quantification strongly depended on the particular experimental conditions. The release kinetics of FGF-2 from the gels could be monitored sensitively by 125I labeling and by ELISA-techniques. The latter method was concluded to be advantageous since it permits the application of unmodified (“native”) growth factors.

Source
Journal of Materials Science : Materials in Medicine 21, 915-923

DOI
http://dx.doi.org/10.1007/s10856-009-3913-z

Published
January 2010
 
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