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Authors
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Kreuzer, L. P. ; Männel, M. J. ; Schubert, J. ; Höller, R.P.M. ; Chanana, M.
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Title
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Enzymatic catalysis at nanoscale: Enzyme-coated nanoparticles as colloidal biocatalysts for polymerization reactions
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Date
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27.10.2017
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Number
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53848
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Abstract
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Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the polymer product. Here, we present a compelling approach for the separation of the two macromolecular species, by taking the catalyst out of the molecular domain and locating it in the colloidal domain, ensuring quasi-homogeneous catalysis as well as easy separation of precious biocatalysts. We report on gold nanoparticles coated with horseradish peroxidase that can catalyze the polymerization of various monomers (e.g., N-isopropylacrylamide), yielding thermoresponsive polymers. Strikingly, these biocatalyst-coated nanoparticles can be recovered completely and reused in more than three independent polymerization cycles, without significant loss of their catalytic activity.
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Publisher
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ACS Omega
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Wikidata
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Citation
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ACS Omega 2 (2017) 7305-7312
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DOI
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https://doi.org/10.1021/acsomega.7b00700
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Tags
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