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Title Hydrogel microvalves as control elements for parallelized enzymatic cascade reactions in microfluidics
Date 10.02.2020
Number 58244
Abstract Compartmentalized microfluidic devices with immobilized catalysts are a valuable tool for overcoming the incompatibility challenge in (bio) catalytic cascade reactions and high-throughput screening of multiple reaction parameters. To achieve flow control in microfluidics, stimuli-responsive hydrogel microvalves were previously introduced. However, an application of this valve concept for the control of multistep reactions was not yet shown. To fill this gap, we show the integration of thermoresponsive poly(N-isopropylacrylamide) (PNiPAAm) microvalves (diameter: 500 and 600 µm) into PDMS-on-glass microfluidic devices for the control of parallelized enzyme-catalyzed cascade reactions. As a proof-of-principle, the biocatalysts glucose oxidase (GOx), horseradish peroxidase (HRP) and myoglobin (Myo) were immobilized in photopatterned hydrogel dot arrays (diameter of the dots: 350 µm, amount of enzymes: 0.13–2.3 µg) within three compartments of the device. Switching of the microvalves was achieved within 4 to 6 s and thereby the fluid pathway of the enzyme substrate solution (5 mmol/L) in the device was determined. Consequently, either the enzyme cascade reaction GOx-HRP or GOx-Myo was performed and continuously quantified by ultraviolet-visible (UV-Vis) spectroscopy. The functionality of the microvalves was shown in four hourly switching cycles and visualized by the path-dependent substrate conversion.
URL https://doi.org/10.3390/MI11020167
Publisher Micromachines
Identifier
Citation Micromachines 11 (2020) 167
DOI https://doi.org/10.3390/MI11020167
Authors Obst, F. ; Beck, A. ; Bishayee, C. ; Mehner, P. J. ; Richter, A. ; Voit, B. ; Appelhans, D.
Tags thermoresponsive hydrogel valves poly(n-isopropylacrylamide) (pnipaam) polydimethylsiloxane (pdms)-on-glass microfluidics enzyme parallelization

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