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Authors
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Perin, G. B.; Moreno, S.; Zhou, Y.; Günther, M.; Boye, S.; Voit, B.; Felisberti, M. I.; Appelhans, D.
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Title
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Construction of membraneless and multicompartmentalized coacervate protocells controlling a cell metabolism-like cascade reaction
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Date
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20.11.2023
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Number
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0
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Abstract
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In recent years, there has been growing attention to designing synthetic protocells, capable of mimicking micrometric and multicompartmental structures and highly complex physicochemical and biological processes with spatiotemporal control. Controlling metabolism-like cascade reactions in coacervate protocells is still challenging since signal transduction has to be involved in sequential and parallelized actions mediated by a pH change. Herein, we report the hierarchical construction of membraneless and multicompartmentalized protocells composed of (i) a cytosol-like scaffold based on complex coacervate droplets stable under flow conditions, (ii) enzyme-active artificial organelles and a substrate nanoreservoir capable of triggering a cascade reaction between them in response to a pH increase, and (iii) a signal transduction component based on the urease enzyme capable of the conversion of an exogenous biological fuel (urea) into an endogenous signal (ammonia and pH increase). Overall, this strategy allows a synergistic communication between their components within the membraneless and multicompartment protocells and, thus, metabolism-like enzymatic cascade reactions. This signal communication is transmitted through a scaffold protocell from an “inactive state” (nonfluorescent protocell) to an “active state” (fluorescent protocell capable of consuming stored metabolites).
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Publisher
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Biomacromolecules
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Wikidata
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Citation
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Biomacromolecules 24 (2023) 5807-5822
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DOI
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https://doi.org/10.1021/acs.biomac.3c00828
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Tags
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