Authors Kaupbayeva, B. ; Boye, S. ; Munasinghe, A. ; Murata, H. ; Matyjaszewski, K. ; Lederer, A. ; Colina, C.M. ; Russell, A.J.
Title Molecular dynamics-guided design of a functional protein-ATRP conjugate that eliminates protein-protein interactions
Date 16.04.2021
Number 59286
Abstract Even the most advanced protein–polymer conjugate therapeutics do not eliminate antibody–protein and receptor–protein recognition. Next-generation bioconjugate drugs will need to replace stochastic selection with rational design to select desirable levels of protein–protein interaction while retaining function. The “Holy Grail” for rational design would be to generate functional enzymes that are fully catalytic with small molecule substrates while eliminating interaction between the protein surface and larger molecules. Using chymotrypsin, an important enzyme that is used to treat pancreatic insufficiency, we have designed a series of molecular chimeras with varied grafting densities and shapes. Guided by molecular dynamic simulations and next-generation molecular chimera characterization with asymmetric flow field-flow fractionation chromatography, we grew linear, branched, and comb-shaped architectures from the surface of the protein by atom-transfer radical polymerization. Comb-shaped polymers, grafted from the surface of chymotrypsin, completely prevented enzyme inhibition with protein inhibitors without sacrificing the ability of the enzyme to catalyze the hydrolysis of a peptide substrate. Asymmetric flow field-flow fractionation coupled with multiangle laser light scattering including dynamic light scattering showed that nanoarmor designed with comb-shaped polymers was particularly compact and spherical. The polymer structure significantly increased protein stability and reduced protein–protein interactions. Atomistic molecular dynamic simulations predicted that a dense nanoarmor with long-armed comb-shaped polymer would act as an almost perfect molecular sieve to filter large ligands from substrates. Surprisingly, a conjugate that was composed of 99% polymer was needed before the elimination of protein–protein interactions.
Publisher Bioconjugate Chemistry
Wikidata Q108626461
Citation Bioconjugate Chemistry 32 (2021) 821-832

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