Authors
|
Constante, G. ; Apsite, I. ; Alkhamis, H. ; Dulle, M. ; Schwarzer, M. ; Caspari, A. ; Synytska, A. ; Salehi, S. ; Ionov, L.
|
Title
|
4D biofabrication using a combination of 3D printing and melt-electrowriting of shape-morphing polymers
|
Date
|
26.08.2021
|
Number
|
59082
|
Abstract
|
We report the fabrication of scroll-like scaffolds with anisotropic topography using 4D printing based on a combination of 3D extrusion printing of methacrylated alginate, melt-electrowriting of polycaprolactone fibers, and shape-morphing of the fabricated object. A combination of 3D extrusion printing and melt-electrowriting allows programmed deposition of different materials and fabrication of structures with high resolution. Shape-morphing allows the transformation of a patterned surface of a printed structure in a pattern on inner surface of a folded object that is used to align cells. We demonstrate that the concentration of calcium ions, the environment media, and the geometrical shape of the scaffold influences shape-morphing that allows it to be efficiently programmed. Myoblasts cultured inside a scrolled bilayer scaffold demonstrate excellent viability and proliferation. Moreover, the patterned surface generated by PCL fibers allow a very high degree of orientation of cells, which cannot be achieved on the alginate layer without fibers.
|
Publisher
|
ACS Applied Materials & Interfaces
|
Wikidata
|
|
Citation
|
ACS Applied Materials & Interfaces 13 (2021) 12767-12776
|
DOI
|
https://doi.org/10.1021/ACSAMI.0C18608
|
Tags
|
|