Authors Engelke, J. ; Boye, S. ; Tuten, B. T. ; Barner-Kowollik, C. ; Barner, L. ; Lederer, A.
Title Critical assessment of the application of multidetection SEC and AF4 for the separation of single-chain nanoparticles
Date 20.10.2020
Number 58984
Abstract The intramolecular chain collapse of linear precursors with systematic variation of molar mass and ligation group density (5, 15, and 30 mol %) into single-chain nanoparticles (SCNPs) was studied by two different separation approaches. The efficiency of size exclusion chromatography with quadruple detection (SEC-D4) was compared to asymmetrical field flow fractionation hyphenated to quintuple detection (AF4-D5) in organic solvent. The application of the unique combination of advanced detection to different separation principles opens up the opportunity to critically evaluate the determination of molar masses and different types of radii for an in-depth understanding of the structural properties affected by the internal folding process. This is achieved by a detailed comparison of assets, drawbacks, and limitations of these approaches based on the systematical screening of different chain lengths and sizes of the precursors and the SCNPs. Furthermore, an alternative strategy for quantitative determination of intramolecular ligation density by a combination of AF4 and UV detection is introduced.
Publisher ACS Macro Letters
Wikidata Q105086316
Citation ACS Macro Letters 9 (2020) 1569-1575

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