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Authors
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Schurig, K. ; Werner, C. ; Zieris, A. ; Hermann, A. ; Freudenberg, U. ; Heidel, S. ; Grimmer, M. ; Storch, A.
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Title
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Neurotropic growth factors and glycosaminoglycan based matrices to induce dopaminergic tissue formation
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Date
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01.10.2015
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Number
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45082
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Abstract
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Current cell replacement therapies in Parkinsons disease (PD) are limited by low survival of transplanted cell and lacking regeneration of neuronal circuitries. Therefore, bioartificial cell carriers and growth/differentiation factors are applied to improve the integration of transplants and maximize newly generated and/or residual dopaminergic function. In this work, biohybrid poly(ethylene glycol) (starPEG)-heparin hydrogels releasing fibroblast growth factor 2 (FGF-2) and glial-derived neurotrophic factor (GDNF) were used to trigger dopaminergic tissue formation by primary murine midbrain cells in vitro. Matrix-delivered FGF-2 enhanced cell viability while release of GDNF had a pro-neuronal/dopaminergic effect. Combined delivery of both factors from the glycosaminoglycan-based matrices resulted in a tremendous improvement in survival and maturation capacity of dopaminergic neurons as obvious from tyrosine hydroxylase expression and neurite outgrowth. The reported data demonstrate that glycosaminoglycan-based hydrogels can facilitate the administration of neurotrophic factors and are therefore instrumental in potential future treatments of PD.
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Publisher
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Biomaterials
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Wikidata
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Citation
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Biomaterials 67 (2015) 205-213
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DOI
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https://doi.org/10.1016/j.biomaterials.2015.07.029
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Tags
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glycosaminoglycan hydrogel neurotrophic growth factor cell replacement neural stem cells
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