Authors Bittrich, E. ; Rodenhausen, K. B. ; Eichhorn, K.-J. ; Hofmann, T. ; Schubert, M. ; Stamm, M. ; Uhlmann, P.
Title Protein adsorption on and swelling of polyelectrolyte brushes: A simultaneous ellipsometry-quartz crystal microbalance study
Date 31.12.2010
Number 24183
Abstract With a coupled spectroscopic ellipsometry-quartz crystal microbalance with dissipation (QCM-D) experimental setup, quantitative information can be obtained about the amount of buffer components (water molecules and ions) coupled to a poly(acrylic acid) (PAA) brush surface in swelling and protein adsorption processes. PAA Guiselin brushes with more than one anchoring point per single polymer chain were prepared. For the swollen brushes a high amount of buffer was found to be coupled to the brush-solution interface in addition to the content of buffer inside the brush layer. Upon adsorption of bovine serum albumin the further incorporation of buffer molecules into the protein-brush layer was monitored at overall electrostatic attractive conditions [below the protein isolectric poimt (IEP)] and electrostatic repulsive conditions (above the protein IEP), and the shear viscosity of the combined polymer-protein layer was evaluated from QCM-D data. For adsorption at the “wrong side” of the IEP an incorporation of excess buffer molecules was observed, indicating an adjustment of charges in the combined polymer-protein layer. Desorption of protein at pH 7.6 led to a very high stretching of the polymer-protein layer with additional incorporation of high amounts of buffer, reflecting the increase of negative charges on the protein molecules at this elevated pH. © 2010 American Vacuum Society
Publisher Biointerphases : An Open Access Journal for the Biomaterials Interfaces Community
Citation Biointerphases : An Open Access Journal for the Biomaterials Interfaces Community 5 (2010) 159 (9 pages)
Tags bovine serum-albumin polymer brushes in-situ optical-properties surfaces layers behavior functionalization environments dissipation

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