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Authors Bonda, U. ; Jaeschke, A. ; Lighterness, A. ; Baldwin, J. ; Werner, C. ; De-Juan-Pardo, E. M. ; Bray, L.
Title 3D quantification of vascular-like structures in z-stack confocal images
Date 18.12.2020
Number 59001
Abstract Optical slice microscopy is commonly used to characterize the morphometric features of 3D cellular cultures, such as in vitro vascularization. However, the quantitative analysis of those structures is often performed on a single 2D maximum intensity projection image, limiting the accuracy of data obtained from 3D cultures. Here, we present a protocol for the quantitative analysis of z stack images, utilizing Fiji, Amira, and WinFiber3D. This protocol facilitates the in-depth examination of vascular-like structures within 3D cell culture models.
Publisher STAR Protocols
Wikidata
Citation STAR Protocols 1 (2020) Article Number: 100180
DOI https://doi.org/10.1016/J.XPRO.2020.100180
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