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Authors
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Lee, G. ; Oh, Y. ; Nam, J.T. ; Ji, S. ; Jang, A.-R. ; Jeong, D.W. ; Kang, M. ; Lee, S.S. ; Chae, S. ; Cho, D. ; Hwang, J.Y. ; Lee, K. ; Lee, J.-O.
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Title
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Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology
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Date
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03.10.2022
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Number
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60817
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Abstract
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In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z-axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.
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Publisher
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Nanotechnology
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Wikidata
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Citation
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Nanotechnology 33 (2022) Article Number: 505101
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DOI
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https://doi.org/10.1088/1361-6528/AC9143
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Tags
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