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Authors
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Zhu, H. ; Liu, S. ; Guo, Z. ; Yan, K. ; Shen, J. ; Zhang, Z. ; Chen, J. ; Guo, Y. ; Liu, L. ; Wu, X.
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Title
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Strong histamine torsion Raman spectrum enables direct, rapid, and ultrasensitive detection of allergic diseases
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Date
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29.10.2021
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Number
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59966
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Abstract
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Allergic diseases are closely related to degranulation and release of histamine and difficult to diagnose because non-allergic diseases also exhibit the same clinical symptoms as allergy. Here, we report direct, rapid, and ultrasensitive detection of histamine using low-frequency molecular torsion Raman spectroscopy. We show that the low-frequency (<200 cm-1) Raman spectral intensities are stronger by one order of magnitude than those of the high-frequency Raman ones. Density functional theory calculation and nuclear magnetic resonance spectroscopy identify the strong spectral feature to be from torsions of carbon-carbon single bonds, which produce large variations of the polarizability densities in the imidazole ring and ethyl amino side chain. Using an omniphobic substrate and surface plasmonic effect of Au@SiO2 nanoparticles, the detection limit (signal-noise ratio >3) of histamine reaches 10-8 g/L in water and 10-6 g/L in serum. This scheme thus opens new lines of inquiry regarding the clinical diagnosis of allergic diseases.
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Publisher
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iScience
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Wikidata
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Citation
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iScience 24 (2021) 103384
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DOI
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https://doi.org/10.1016/J.ISCI.2021.103384
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Tags
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