Authors Müller, M. ; Rieser, Th. ; Dubin, P. ; Lunkwitz, K.
Title Selective Interaction Between Proteins and the Outermost Surface of Polyelectrolyte Multilayers: Influence of the Polyanion Type, pH and Salt
Date 11.04.2001
Number 9271
Abstract Protein adsorption was studied by in-situ ATR-FT-IR spectroscopy of consecutively deposited polyelectrolyte multilayer systems terminated either with poly(ethyleneimine) (PEI) or polyanions, such as poly-(acrylic acid) (PAC), poly(maleic acid-co-propylene) (PMA-P) or poly(vinyl sulfate) (PVS). The influence of the polyanion type, pH and ionic strength was investigated. Negatively charged human serum albumin (HSA) was strongly repelled by multilayers terminated with weak polyanions (PAC, PMA-P), whereas moderate attraction was observed for those terminated with the strong polyanion PVS. Changing the pH from 7.4 to 5 resulted in enhanced HSA adsorption onto PAC-terminated multilayers. An increase in ionic strength diminished the attractive HSA adsorption onto PEI-terminated multilayers. For the PEI/PAC system, the biomedically relevant adsorption of human fibrinogen (FGN) is determined via its isoelectric point in accordance with three other proteins.
Publisher Macromolecular Rapid Communications
Citation Macromolecular Rapid Communications 22 (2001) 390-395
Tags situ atr-ftir spectroscopy adsorption layers

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