Authors Constante, G. ; Apsite, I. ; Alkhamis, H. ; Dulle, M. ; Schwarzer, M. ; Caspari, A. ; Synytska, A. ; Salehi, S. ; Ionov, L.
Title 4D biofabrication using a combination of 3D printing and melt-electrowriting of shape-morphing polymers
Date 26.08.2021
Number 59082
Abstract We report the fabrication of scroll-like scaffolds with anisotropic topography using 4D printing based on a combination of 3D extrusion printing of methacrylated alginate, melt-electrowriting of polycaprolactone fibers, and shape-morphing of the fabricated object. A combination of 3D extrusion printing and melt-electrowriting allows programmed deposition of different materials and fabrication of structures with high resolution. Shape-morphing allows the transformation of a patterned surface of a printed structure in a pattern on inner surface of a folded object that is used to align cells. We demonstrate that the concentration of calcium ions, the environment media, and the geometrical shape of the scaffold influences shape-morphing that allows it to be efficiently programmed. Myoblasts cultured inside a scrolled bilayer scaffold demonstrate excellent viability and proliferation. Moreover, the patterned surface generated by PCL fibers allow a very high degree of orientation of cells, which cannot be achieved on the alginate layer without fibers.
Journal ACS Applied Materials & Interfaces
Citation ACS Applied Materials & Interfaces 13 (2021) 12767-12776

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