Authors Bray, L. ; Binner, M. ; Freudenberg, U. ; Werner, C.
Title Hydrogel-based in vitro models of tumor angiogenesis
3D Cell Culture, Koledova, Z., ed.
Date 31.12.2017
Number 54736
Abstract In situ forming hydrogels prepared from multi-armed poly(ethylene glycol) (PEG), glycosaminoglycans (GAG) and various peptides enable the development of advanced three dimensional (3D) culture models. Herein, we report methods for the PEG-GAG gel-based 3D co-cultivation of human umbilical vein endothelial cells, mesenchymal stromal cells, and different cancer cell lines. The resulting constructs allow for the exploration of interactions between solid tumors with 3D vascular networks in vitro to study the mechanistic aspects of cancer development and to perform drug testing. From 3D cell culture : methods and protocols; Part of the Methods in Molecular Biology book series (MIMB, volume 1612) ISBN 978-1-4939-7019-3
Publisher Humana Press
Citation Humana Press 2017 (2017) 39-63

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