Authors Gorzkiewicz, M. ; Sztandera, K. ; Jatczak-Pawlik, I. ; Zinke, R. ; Appelhans, D. ; Klajnert-Maculewicz, B. ; Pulaski, L.
Title Terminal sugar moiety determines immunomodulatory properties of poly(propyleneimine) glycodendrimers
Date 23.03.2018
Number 54588
Abstract Poly(propyleneimine) dendrimers fully surface-modified with disaccharide moieties (maltose, cellobiose, and lactose) designed to mimic natural lectin receptor ligands were tested for their bioactivity in two myeloid cell lines: THP-1 and HL-60. Depending on the sugar modification, we observed variable activation of NF-·B, AP-1, and NF-AT signaling pathways: lactose-coated dendrimers had the strongest impact on marker gene expression and most signaling events with the notable exception of NF-·B activation in THP-1 cells. The two cell lines showed an overall similar pattern of transcription factor and gene expression activation upon treatment with glycodendrimers, suggesting the involvement of galectin and C-type lectin receptor types. An important result of this action was the overexpression of CD40 and IL8 genes, potentially leading to an activated, proinflammatory phenotype in the monocyte/macrophage cell lineage. These pharmacodynamic characteristics of glycodendrimers need to be taken into account during their pharmaceutical applications both in drug delivery and direct immunomodulation.
Publisher Biomacromolecules
Citation Biomacromolecules 19 (2018) 1562-1572

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