Authors Thomas, A. K. ; Wieduwild, R. ; Zimmermann, R. ; Lin, W. ; Friedrichs, J. ; Bickle, M. ; Fahmy, K. ; Werner, C. ; Zhang, Y.
Title Layer-by-layer assembly of heparin and peptide-polyethylene glycol conjugates to form hybrid nanothin films of biomatrices
Date 16.04.2018
Number 53925
Abstract We investigated the utility of a heparin/peptide-polyethylene glycol conjugate system to build layer-by-layer (LbL) structures, to assemble tailored multilayer-biomatrices for cell culture. The LbL assembly balances the advantages of polyelectrolyte systems and protein-based systems. Human umbilical vein endothelial cells showed distinct responses to the film thickness and structure; the presence, density, and spatial arrangement of a cell adhesion ligand within the nanothin film; and the pretreatment of the film with morphogens. The LbL technique presents a versatile tool for modifying cell culture substrates with defined and diverse biochemical and structural features, for investigating cell–material interactions.
Publisher ACS Applied Materials & Interfaces
Citation ACS Applied Materials & Interfaces 10 (2018) 14264-14270
Tags biomimetic extracellular matrix heparin layer-by-layer nano thin

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