Authors Renner, L. ; Zan, J. ; Hu, L. I. ; Martinez, M. ; Resto, J.P. ; Siegel, A. C. ; Torres, C. ; Hall, S. B. ; Slezak, T. R. ; Nguyen, T. H. ; Weibel, D. B.
Title Detection of ESKAPE bacterial pathogens at the point-of care using isothermal DNA-based assays in a portable, de-gas microfluidic diagnostic assay platform
Date 01.02.2017
Number 52427
Abstract An estimated 1.5B microbial infections occur globally each year and result in ~4.6M deaths. A technology gap associated with commercially available diagnostic tests in remote and underdeveloped regions prevents timely pathogen identification for effective antibiotic chemotherapies for infected patients. The result is a trial-and-error approach that is limited in effectiveness and increases risk for patients while contributing to antimicrobial drug resistance and reduces the lifetime of antibiotics. This manuscript addresses this important diagnostic technology gap by describing a low cost, portable, rapid, and easy-to-use microfluidic cartridge-based system for detecting the ESKAPE bacterial pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp) that are most commonly associated with antibiotic resistance. The point-of-care molecular diagnostic system consists of a vacuum-degassed microfluidic cartridge pre-loaded with lyophilized recombinase polymerase amplification (RPA) assays and a small, portable battery-powered electronic incubator/reader. The isothermal RPA assays detect the targeted ESKAPE pathogens with high sensitivity (e.g., a limit of detection of ~10 nucleic acid molecules) that is comparable to current PCR-based assays and offer advantages in power consumption, engineering, and robustness, which are three critical elements required for the point-of-care setting.
Publisher Environmental Microbiology
Citation Environmental Microbiology 83 (2017) e02449-16

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