Authors Ciepluch, K. ; Nyström, B. ; Appelhans, D. ; Zablocka, M. ; Bryszewska, M. ; Majoral, J. P.
Title Effect of dendritic polymers on a simple model biological membrane
Date 01.08.2015
Number 47581
Abstract The higher disruption of lipid bilayer and higher cytotoxicity caused by nanoparticles are often linked to the aggregation process of the particles onto the biological membrane of the cell. To understand how nanoparticles behave when they meet a biological membrane it is important to consider their potential applications in biomedical science. In spite of many biological applications of dendritic scaffolds, a major problem of their cationic exponents is the disruptive biological properties against cell membranes leading to toxicity. Up to now many studies about biological and biochemical effects on membranes have emerged. Therefore, we have investigated the interaction of different kinds of dendritic scaffols on a simple biological membrane model: a small cationic dendrimer with viologen units, hyperbranched poly(ethyleneimine) (PEI), and maltosylated PEI. Our main aim was to check how different types of dendritic scaffolds can affect the model membrane. To probe this effect, we employed dynamic light scattering (DLS), zeta potential, fluorescence, and transmission electron microscopy (TEM) techniques. Results from this work facilitate our understanding of interaction of dendritic nanoparticles with biological membranes. This investigation can help use to explain the toxicological effect of these nanoparticles. As a model of a biological membrane, we use negatively charged liposomes, consisting of a mixture of lipids: 90 % DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and 10 % DPPG (1,2-Dipalmitoyl-sn-glycero-3-phosphoglycerol).
Publisher Journal of Polymer Research
Citation Journal of Polymer Research 22 (2015) 184, 10 Seiten
Tags dendritic polymers liposomes dynamic light scattering membrane model

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