Authors
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Hauptmann, N. ; Pion, M. ; Munoz Fernández, M.-Á. ; Komber, H. ; Werner, C. ; Voit, B. ; Appelhans, D.
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Title
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Ni(II)-NTA modified poly(ethylene imine) glycopolymers: Physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides
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Date
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11.03.2013
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Number
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37562
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Abstract
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Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5–6.0, and also up to 50?mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.
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Publisher
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Macromolecular Bioscience
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Wikidata
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Q46356113
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Citation
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Macromolecular Bioscience 13 (2013) 531-538
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DOI
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https://doi.org/10.1002/mabi.201200449
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Tags
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dendritic cells dendritic glycopolymers nta ligands poly(ethylene imine) polyplexes dendritic cells tagged proteins hyperbranched-pei purification delivery immunotherapy complexes dna
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