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Authors
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Friedrichs, J. ; Zieris, A. ; Prokoph, S. ; Werner, C.
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Title
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Quantifying the effect of covalently immobilized enzymes on biofilm formation by atomic force microscopy-based single-cell force spectroscopy
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Date
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07.09.2012
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Abstract
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A novel atomic force microscopy-based single-cell force spectroscopy assay to quantify the adhesion of bacterial cells to surfaces was developed. The assay was applied to quantify the effect of two biofilm-degrading enzymes, the protease Subtilisin A and glycoside hydrolase cellulase, on the attachment of the biofilm-forming bacterial strain Cobetia marina. Insights on the mechanism of the initial adhesion and on the nature of the adhesion-mediating molecules were gained. The assay can be easily adapted to various other substrates, different bacterial strains and other fouling species (e.g., algae and diatoms).
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Journal
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Macromolecular Rapid Communications 33 (2012) 1453-1458
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DOI
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https://doi.org/10.1002/marc.201200359
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