Authors Mkandawire, M. ; Pohl, A. ; Gubarevich, T. ; Lapina, V. ; Appelhans, D. ; Rödel, G. ; Pompe, W. ; Schreiber, J. ; Opitz, J.
Title Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells
Date 07.10.2009
Number 19063
Abstract Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4th-generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Publisher Journal of Biophotonics
Citation Journal of Biophotonics 2 (2009) 596-606

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