Authors Layman, J. M. ; Borgerding, E. M. ; Williams, S. R. ; Appelhans, D. ; Voit, B. ; Long, T. E.
Title Elucidating structure-property relationships in polycation-mediated gene delivery
Date 12.05.2008
Abstract PMSE 185 - Drug Delivery Systems <br /><br />Nonviral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators have shown that transfer efficiencies are considerably lower when compared to viral vectors. Our work aims to fundamentally understand the underlying structure-transfection relationships involved in polycation-mediated gene delivery. Our efforts have focused on the influence of molecular weight, macromolecular topology, oligosaccharide modifications, and charge density on the overall transfection activity in vitro. Several families of polycations were synthesized in order to correlate chemo-physical characterization with transfection results. We have found that seemingly small changes in the structure of cationic polyelectrolytes can have profound consequences on their transfection activity.
Journal Polymeric Materials: Science and Engineering 98 (2008) 303-304

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