Authors Müller, M. ; Reihs, T. ; Keßler, B. ; Adler, H. J. ; Lunkwitz, K.
Title Multilayers Consisting of Oriented Charged a-Helical Polypeptides
Date 14.05.2004
Number 11579
Abstract Polyelectrolyte Multilayers (PEMs) consisting of cationic <IMGSRC="/giflibrary/12/agr.gif"BORDER="0">-helical poly(L-lysine) (PLL) and optionally anionic poly(vinysulfate) (PVS) (i) or <IMGSRC="/giflibrary/12/agr.gif"BORDER="0">-helical poly(L-glutamic acid) (PLG) (ii) were deposited at substrates texturized by parallel nanoscopic surface grooves, respectively. Using dichroic Attenuated Total Reflexion Fourier Transform Infrared (ATR-FTIR) spectroscopy the consecutive deposition, conformation and macromolecular order of stiff polyelectrolytes within PEMs were studied. From the dichroic ratios of the Amide I and Amide II bands order parameters S <IMGSRC="/giflibrary/12/ges.gif"BORDER="0"> 0.6 (S = 1 for high, S = 0 for low order) were obtained suggesting a significant alignment of charged <IMGSRC="/giflibrary/12/agr.gif"BORDER="0">-helical polypeptides in PEMs. For the PEM consisting of PLL/PVS the deposited amount as well as the order parameter S significantly depended on the molecular weight (contour length) of PLL. Furthermore, the related opening angle <IMGSRC="/giflibrary/12/ggr.gif"BORDER="0"> of a model cone consisting of <IMGSRC="/giflibrary/12/agr.gif"BORDER="0">-helical PLL rods was proven to be a function of both contour length and width of the confining surface grooves. AFM pictures on PEM-PLL/PVS showed anisotropically oriented worm-like structures As a second system PEMs of PLL and PLG, both in the <IMGSRC="/giflibrary/12/agr.gif"BORDER="0">-helical conformation, are introduced. A high order parameter of both spectroscopically indistinguishable polypeptides was found. A model of aligned rod/coil (i) and rod/rod (ii) structures was proposed. Finally, multilayers of stiff conductive polymers like polyaniline (PANI) alternating with poly(acrylic acid) (PAC) are introduced. Preliminary results on their deposition and alignment are given.</T<br />D></TR></TABLE><HRSIZE="1"WIDTH="25%"ALIGN="left"><P><TABLEBORDER="0"WIDTH="100%"><TR><TDclass="mainSectionHeader"><Aname="doi">Digital Object Identifier (DOI)</A>
Publisher Macromolecular Symposia
Citation Macromolecular Symposia 211 (2004) 217-233

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