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Title Engineering bacterial shape using soft-matter microchambers
Date 01.03.2019
Number 56012
Abstract This article describes the design and fabrication of microchambers that are used for the study of bacterial cells. The design allows for the confinement and precise manipulation of bacterial cell shape. The application of fluorescent dyes and fluorescent proteins enables the precise analysis of the localization of biomolecules within confined bacterial cell. This article also outlines three methods to engineer cell shape from a filamentous cell type and from spheroplasts without a cell wall using soft lithography–based technologies. © 2018 by John Wiley & Sons, Inc.
Publisher Current Protocols
Identifier
Citation Current Protocols 11 (2019) e59
DOI https://doi.org/10.1002/cpch.59
Authors Renner, L.
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