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Title Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris
Date 25.10.2013
Number 40622
Abstract This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to a-chitin, known to be present in other animals, than to ß-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.
Publisher Journal of Structural Biology
Identifier
Citation Journal of Structural Biology 183 (2013) 474-483
DOI http://dx.doi.org/10.1016/j.jsb.2013.06.015
Authors Ehrlich, H. ; Kaluzhnaya, O. V. ; Brunner, E. ; Tsurkan, M. ; Ereskovsky, A. ; Ilan, M. ; Tabachnick, K. R. ; Bazhenov, V. V. ; Paasch, S. ; Kammer, M. ; Born, R. ; Stelling, A. ; Galli, R. ; Belikov, S. ; Petrova, O. V. ; Sivkov, V. N. ; Vyalikh, D. V. ;
Tags sponges skeleton chitin chitin synthase bioanalytics

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