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Authors
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Moreno, S. ; Hübner, H. ; Effenberg, C. ; Boye, S. ; Ramuglia, A. ; Schmitt, D. ; Voit, B. ; Weidinger, I.M. ; Gallei, M. ; Appelhans, D.
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Title
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Redox- and pH-responsive polymersomes with ferrocene moieties exhibiting peroxidase-like, chemoenzymatic activity and H2O2-responsive release behavior
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Date
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10.10.2022
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Number
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60932
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Abstract
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The development of compartments for the design of cascade reactions in a local space requires a selective spatiotemporal control. The combination of enzyme-loaded polymersomes with enzymelike units shows a great potential in further refining the diffusion barrier and the type of reactions in nanoreactors. Herein, pH-responsive and ferrocene-containing block copolymers were synthesized to realize pH-stable and multiresponsive polymersomes. Permeable membrane, peroxidase-like behavior induced by the redox-responsive ferrocene moieties and release properties were validated using cyclovoltammetry, dye TMB assay, and rupture of host–guest interactions with ß-cyclodextrin, respectively. Due to the incorporation of different block copolymers, the membrane permeability of glucose oxidase-loaded polymersomes was changed by increasing extracellular glucose concentration and in TMB assay, allowing for the chemoenzymatic cascade reaction. This study presents a potent synthetic, multiresponsive nanoreactor platform with tunable (e.g., redox-responsive) membrane properties for potential application in therapeutics.
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Publisher
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Biomacromolecules
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Wikidata
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Q116313199
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Citation
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Biomacromolecules 23 (2022) 4655-4667
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DOI
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https://doi.org/10.1021/ACS.BIOMAC.2C00901
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Tags
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