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Authors
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Bucatariu, F. ; Simon, F. ; Fundueanu, G. ; Dragan, E. S.
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Title
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Trypsin immobilization onto silica/(polyelectrolyte)n hybrid microparticles
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Date
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31.12.2011
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Number
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32082
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Abstract
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A novel route to synthesize silica//poly(vinyl amine) (silica//PVAm) and silica//chitosan (silica//CHI) hybrid microparticles containing trypsin attached on the surface was developed. PVAm and CHI were adsorbed from salt-free aqueous solution onto silica microparticles with particle and pore diameter of 40-60 ìm, and 100 nm, respectively.<br />Then, the enzyme was covalently grafted to the surface via a glutaraldehyde (GA) cross-linker. Alternatively, a thick layer was chemisorbed by cross-linking two polyelectrolyte layers with GA. X-ray photoelectron spectroscopy (XPS), potentiometric and polyelectrolyte titrations were employed to obtain information about the polyelectrolyte adsorption and multilayer formation, as well as the amount of the trypsin that was covalently bound to the functionalized surfaces.<br />The study showed that only PVAm formed a double layer onto silica microparticles, while the amount of grafted enzyme did not significantly vary with the adsorbed amount and polyelectrolyte type.
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Publisher
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Buletinul Institutului Politehnic din Ia¸si / Sec¸tia Chimie ¸si inginerie chimica
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Wikidata
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Citation
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Buletinul Institutului Politehnic din Ia¸si / Sec¸tia Chimie ¸si inginerie chimica 57 (2011) 109-116
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DOI
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http://www.tuiasi.ro/facultati/ic/index.php?page=593
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Tags
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