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Authors
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Bucatariu, F. ; Simon, F. ; Bellmann, C. ; Fundueanua, G. ; Dragan, E. S.
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Title
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Stability under flow conditions of trypsin immobilized onto poly(vinyl amine) functionalized silica microparticles
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Date
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30.04.2012
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Number
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29995
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Abstract
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A novel route to synthesize organic/inorganic hybrid microparticles containing trypsin attached on the surface was developed. Poly(vinyl amine) (PVAm) with low (15,000 g mol-1) and high (340,000 g mol-1) molar mass was adsorbed from salt-free aqueous solution onto silica microparticles with particle diameter of 4060 µm. Then, the enzyme was covalently bound to the surface via glutaraldehyde (GA) as cross-linker. X-ray photoelectron spectroscopy (XPS), potentiometric and polyelectrolyte titrations, and electrokinetic measurements were employed to obtain information about the polyelectrolyte adsorption and multilayer formation, as well as the amount of trypsin that has been covalently bound to the PVAm-functionalized surface. The study showed that the stability of the organic layer carrying the enzyme has been strongly influenced by the molar mass of the PVAm under the streaming fluid condition. The amount of the attached enzyme did not significantly vary with the adsorbed amount of PVAm.
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Publisher
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Colloids and Surfaces A: Physicochemical and Engineering Aspects
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Wikidata
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Citation
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Colloids and Surfaces A: Physicochemical and Engineering Aspects 399 (2012) 71-77
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DOI
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https://doi.org/10.1016/j.colsurfa.2012.02.030
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