Authors Schurig, K. ; Werner, C. ; Zieris, A. ; Hermann, A. ; Freudenberg, U. ; Heidel, S. ; Grimmer, M. ; Storch, A.
Title Neurotropic growth factors and glycosaminoglycan based matrices to induce dopaminergic tissue formation
Date 01.10.2015
Number 45082
Abstract Current cell replacement therapies in Parkinsons disease (PD) are limited by low survival of transplanted cell and lacking regeneration of neuronal circuitries. Therefore, bioartificial cell carriers and growth/differentiation factors are applied to improve the integration of transplants and maximize newly generated and/or residual dopaminergic function. In this work, biohybrid poly(ethylene glycol) (starPEG)-heparin hydrogels releasing fibroblast growth factor 2 (FGF-2) and glial-derived neurotrophic factor (GDNF) were used to trigger dopaminergic tissue formation by primary murine midbrain cells in vitro. Matrix-delivered FGF-2 enhanced cell viability while release of GDNF had a pro-neuronal/dopaminergic effect. Combined delivery of both factors from the glycosaminoglycan-based matrices resulted in a tremendous improvement in survival and maturation capacity of dopaminergic neurons as obvious from tyrosine hydroxylase expression and neurite outgrowth. The reported data demonstrate that glycosaminoglycan-based hydrogels can facilitate the administration of neurotrophic factors and are therefore instrumental in potential future treatments of PD.
Publisher Biomaterials
Citation Biomaterials 67 (2015) 205-213
Tags glycosaminoglycan hydrogel neurotrophic growth factor cell replacement neural stem cells

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