Authors Poitz, D. ; Stölzel, F. ; Arabanian, L. ; Friedrichs, J. ; Docheva, D. ; Schieker, M. ; Fierro, F. ; Platzbecker, U. ; Ordemann, R. ; Werner, C. ; Bornhäuser, M. ; Strasser, R. H. ; Ehninger, G. ; Illmer, T.
Title MiR-134-mediated b1 integrin expression and function in mesenchymal stem cells
Date 07.11.2013
Number 36022
Abstract The composition of the hematopoietic stem cell (HSC) niche within the bone marrow is highly dynamic, tightly regulated, and of importance for various HSC properties. Integrins are important molecules within this niche that influence those properties through the interactions of HSCs and mesenchymal stem cells (MSCs). Here we investigated the function of miR-134 in integrin regulation in MSCs. In MSCs, miR-134 post-transcriptionally regulated ß1 integrin expression. This negative regulation of ß1 integrin was mediated by the binding of miR-134 to its 3' untranslated region, which contains two conserved binding sites for miR-134. The miR-134-mediated silencing of ß1 integrin in MSCs was shown by atomic force microscopy to decrease the adhesion of 32D cells to MSCs transfected with miR-134. Furthermore, the adhesion of MSCs to fibronectin was reduced after transfection with miR-134. MSCs from patients with myelodysplastic syndrome (MDS) revealed highly significant miR-134 overexpression compared with MSCs from healthy bone marrow donors. MSCs from MDS patients showed lower ß1 integrin protein, but not lower mRNA, expression, suggesting post-transcriptional regulation. The present study demonstrates miR-134-mediated negative regulation of ß1 integrin that influences cell adhesion to and of MSCs. These results further contribute to our understanding of the complexity of MDS.
Publisher Biochimica et Biophysica Acta : BBA - Molecular cell research
Citation Biochimica et Biophysica Acta : BBA - Molecular cell research 1833 (2013) 3396-3404
Tags mesenchymal stem cells microrna mir-134 ß1 integrin cd29 scp-1 mds myelodysplastic syndrome

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