Authors Salchert, K. ; Streller, U. ; Pompe, T. ; Herold, N. ; Grimmer, M. ; Werner, C.
Title In vitro Reconstitution of Fibrillar Collagen Type I Assemblies at Reactive Polymer Surfaces
Date 10.08.2004
Number 12155
Abstract The reconstitution of fibrillar collagen and its assemblies with heparin and hyaluronic acid was studied invitro. Fibril formation kinetics were analyzed by turbidity and depletion measurements in solutions containingvaried concentrations of collagen and glycosaminoglycans. Fibril-forming collagen solutions were furtherapplied for the coating of planar substrates which had been modified with alternating maleic anhydridecopolymer films before. The immobilized collagen assemblies were characterized with respect to the depositedamount of protein using ellipsometry and acidic hydrolysis/HPLC-based amino acid analysis, respectively.AFM, SEM, and cLSM were utilized to gain information on structural features and patterns formed bysurface-attached fibrils depending on the initial solution concentrations of collagen. The results revealedthat the addition of heparin and hyaluronic acid affected both the fibril dimensions and the meshworkcharacteristics of the surface-bound fibrils.
Publisher Biomacromolecules
Citation Biomacromolecules 5 (2004) 1340-1350
Tags cross-linked collagen immobilization invitro fibrillogenesis heparin acid matrices protein cells

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