Authors
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Ehrlich, H. ; Kaluzhnaya, O. V. ; Brunner, E. ; Tsurkan, M. ; Ereskovsky, A. ; Ilan, M. ; Tabachnick, K. R. ; Bazhenov, V. V. ; Paasch, S. ; Kammer, M. ; Born, R. ; Stelling, A. ; Galli, R. ; Belikov, S. ; Petrova, O. V. ; Sivkov, V. N. ; Vyalikh, D. V. ;
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Title
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Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris
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Date
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25.10.2013
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Number
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40622
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Abstract
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This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to a-chitin, known to be present in other animals, than to ß-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.
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Publisher
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Journal of Structural Biology
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Wikidata
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Citation
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Journal of Structural Biology 183 (2013) 474-483
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DOI
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https://doi.org/10.1016/j.jsb.2013.06.015
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Tags
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sponges skeleton chitin chitin synthase bioanalytics
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