Authors
|
Himmel, H.M. ; Pietsch, M. ; Streller, U. ; Graf, E.M. ; Ravens, U.
|
Title
|
Changes in morphology and inward rectifier currents in human atrial myocytes depend on culture conditions
|
Date
|
31.12.2002
|
Number
|
10042
|
Abstract
|
Human atrial myocytes were cultured under systematically varied conditions in order to obtain stable cells for future gene manipulation. Transient (Ito) and sustained outward current (Iso), and voltage- and muscarinic receptor-activated inward rectifier K+ currents (IK1, IK,ACh) were measured in freshly isolated cells and after 5 days in culture. Myocytes were grown on polylysin or laminin in medium with or without 10 % serum (medium+S, medium-S). Cultured myocytes dedifferentiated to a greater extent in medium+S than medium-S, but independent of the chemical nature of the adherence surface. Apparent surface area increased in medium+S, whereas membrane capacitance declined under all culture conditions. Ito of myocytes cultured in medium-S was increased. Myocytes grown on polylysin and laminin exhibited reduced IK1 current density. Under all culture conditions, IK,ACh was attenuated with carbachol but hardly affected with sphingosine-1-phosphate as agonists. In conclusion, morphological and electrophysiological changes depended on serum in the culture medium rather than on adherence surface being coated with laminin or polylysin.
|
Publisher
|
Basic Research in Cardiology
|
Wikidata
|
|
Citation
|
Basic Research in Cardiology 97 (2002) 434-444
|
DOI
|
https://doi.org/10.1007/s00395-002-0375-6
|
Tags
|
|