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Authors
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Kräter, M. ; Jacobi, A. ; Otto, O. ; Tietze, S. ; Müller, K. ; Biehain, U. ; Palm, U. ; Zinna, V. ; Wobus, M. ; Chavakis, T. ; Werner, C. ; Bornhäuser, M.
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Title
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Bone marrow niche-mimetics modulate HSPC function via integrin signaling
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Date
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31.05.2017
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Number
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53168
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Abstract
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The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, <br />the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-<br />mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction. Seeding freshly isolated HSPCs adherent (AT) and non-<br />adherent (SN) cells were found. We detected enhanced expansion and active migration of AT-cells mediated by ECM incorporated stromal derived factor one. Probing cell mechanics, AT-cells displayed <br />naïve cell deformation compared to SN-cells indicating physical recognition of ECM material properties by focal adhesion. Integrin aIIb (CD41), aV (CD51) and ß3 (CD61) were found to be induced. Signaling <br />focal contacts via ITGß3 were identified to facilitate cell adhesion, migration and mediate ECM-physical cues to modulate HSPC function.
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Publisher
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Scientific Reports
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Wikidata
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Citation
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Scientific Reports 7 (2017) ID2549
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DOI
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https://doi.org/10.1038/s41598-017-02352-5
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