Responsive Nano-Biointerfaces

Since controlling of surface topography and chemical functionality on a nanometer scale is crucial for many biomedical applications the increasing requirement on the design of ultimate switchable biointerfaces exists. Our research is focused on nano-engineering in order to establish smart nano-biointerfaces based on adaptive and stimuli responsive polymer brush surfaces with multiple functions so that they are able to modify their interactions with cells and biomolecules sensitive to different physical or chemical stimuli. Such behavior may imitate dynamic properties of natural biological systems regulated by several different stimuli.
Another key aspect of our work is the control of interfacial processes via nano-biointerfaces generated from well-defined biomimetic and tunable polymer brush systems exhibiting functional moieties in order to better understand protein/material and cell/material interactions at the submolecular level. Nonspecific biomolecule adsorption using enzymes, growth factors or peptides onto nano-biointerfaces may be essential for achieving versatile biomedical cues. Simultaneously, bioconjugation like specific biomolecule immobilization or the use of click chemistry under maintaining the molecules biofunction is introduced aiming to create a high-performance nano-biointerface. Moreover, the ability to modulate biomolecule activity, protein immobilisation, and cell adhesion at the liquid-solid interface is important in a variety of biological and medical applications. For example it is suggested that using 3D technology for cell culture testing might result in more realistic data than traditional 2D cell culture technology because the native cellular environment is 3D. Therefore there is a need to extend planar, micro- and nanostructured biomedical devices to the third dimension. Our results indicate that a precisely designed polymer brush functioned as an intelligent cell separating interface by utilizing the intrinsic cell detachment properties of individual cells.