Adhesive interactions of cells with their environment can trigger a multitude of desired, but also undesired processes. An undesired, adhesion-associated process is the deposition of microorganisms and their extracellular polymeric substrates on man-made surfaces, so called biofouling or biofilm formation. This phenomenon can occur in an extremely wide range of situations, from the colonization of medical- and process devices to the production of ultra-pure, drinking and process water and the fouling of ship hulls, pipelines and reservoirs. In this context we focus on the development, characterization and application of well-characterized surfaces with antifouling properties. More precisely, we investigate the influence of biofilm-degrading enzymes, covalently immobilized onto maleic anhydride copolymer films, on the adhesion of major marine foulers and bacteria associated with hospital-acquired infections.