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Authors Janaszewska, A. ; Studzian, M. ; Petersen, A. ; Ficker, M. ; Paolucci, V. ; Christensen, J. B. ; Tomalia, D. A. ; Klajnert-Maculewicz, B.
Title Modified PAMAM dendrimer with 4-carbomethoxypyrrolidone surface groups-its uptake, efflux, and location in a cell
Date 01.11.2017
Number 54325
Abstract Traditional amine terminated PAMAM dendrimers may be readily surface engineered by a facile one-pot conversion with dialkyl itaconate esters into 4-carbomethoxypyrrolidone terminated PAMAM (G = 0-4) dendrimers. These terminated dendrimers are uniquely characterized by exhibiting blue fluorescence emissions (·exc = 370 nm, ·maxem = 440 nm). Thanks to this property they can be directly analyzed by confocal microscopy and flow cytometry without additional fluorescence labeling, treatment of dendrimers with chemicals or adjusting pH. These intrinsically fluorescent dendrimers were shown to be very effective for assessing important biological cell features such as cellular entry, intracellular trafficking/localization and efflux properties. For example, all tested cell lines (e.g., B14, BRL-3A, and mHippoE-18) rapidly accumulated PAMAM-pyrrolidone dendrimer. The BRL-3A cell line exhibited both cytoplasmic and nuclear localization patterns; whereas in B14 cells and mHippoE-18 cells, the blue dendrimer fluorescence could only be detected in intracellular endosome-like structures. The dendrimer was observed to be released from all three cell lines during the first 24 h; however, efflux was substantially slower from the B-14 cell line. The highest efflux rate was observed for the mHippoE-18 cells. This first successful biological experiment opens a broad spectrum of using these dendrimers as new bioimaging agents for extensive biological cell characterizations.
Publisher Colloids and Surfaces B: Biointerfaces
Wikidata
Citation Colloids and Surfaces B: Biointerfaces 159 (2017) 211-216
DOI https://doi.org/10.1016/j.colsurfb.2017.07.052
Tags pamam-pyrrolidone dendrimer intrinsic fluorescence uptake bio-imaging

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