Authors
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Burkert, S. ; Bittrich, E. ; Kuntzsch, M. ; Müller, M. ; Eichhorn, K.-J. ; Uhlmann, P. ; Stamm, M.
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Title
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Protein resistance of PNIPAAm brushes: Application to switchable protein adsorption
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Date
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27.01.2010
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Number
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20582
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Abstract
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Protein adsorption, as the primary process occurring when a foreign surface comes into contact with a biosystem, was studied on thin polymer brush films consisting of poly(N-isopropylacrylamide) (PNIPAAm) and poly(2-vinylpyridine) (P2VP). These films were prepared by the grafting to method. The protein resistance of stimuli responsive PNIPAAm-brushes toward serum albumin was recorded and compared with protein adsorption on P2VP brushes. To achieve a better understanding of protein resistance, PNIPAAm brushes with different molecular weights were investigated below and above the lower critical solution temperature of 32 °C. To use these findings for the adjustment and switching of protein adsorption, in a first attempt the adsorption on a mixed brush system consisting of PNIPAAm and P2VP chains was studied. This system showed temperature-dependent adsorption behavior due to the presence of PNIPAAm, representing a smart surface with stimuli-responsive changes in the physicochemical surface properties. With this mixed brush, the adsorbed amount of protein could be controlled, depending on composition and the temperature of the surroundings.
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Publisher
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Langmuir
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Wikidata
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Citation
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Langmuir 26 (2010) 1786-1795
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DOI
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https://doi.org/10.1021/la902505q
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Tags
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human serum-albumin polymer brushes polyelectrolyte brushes model proteins solid-surfaces in-situ layers ellipsometry interface behavior
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